Part:BBa_K1645999:Design

Dual Color Plasmid (RFP and GFP)

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 1708
  Illegal AgeI site found at 1820
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 706
  

Design Notes

First BBa_J04450 was cloned into BBa_I20260 using XbaI and PstI. Then the backbone was changed from pSB3K3 into pSB1C3.

Source

This part is constructed using two existing registry parts, BBa_I20260, BBa_J04450.